Sickle cell disease including Hb SS, Hb SC, Hb Sβ thalassaemia, Hb S/D-Punjab, Hb S/O-Arab, Hb S/Lepore
Beta thalassaemia major
Beta thalassaemia/delta beta thalassaemia
Beta thalassaemia/Hb Lepore
Beta thalassaemia/Hb E
Hb Barts Hyrops Fetalis
Hb Lepore/delta beta thalassaemia
Hb Lepore homozygous
Presence of heparin anticoagulant will inhibit PCR applications.
Samples must be clearly labelled with the patients first name, surname, D.O.B, hospital number and the date the sample was taken. The details on the sample must correspond to the request form. Unlabelled samples will not be accepted.
CVS samples must be cleaned by a cytogenetics department prior to sending.
A signed consent form must accompany the sample. Failure to do so will delay sample processing and reporting.
Chorionic villus sampling (CVS) 9-16 weeks
Samples should be sorted at source in order to remove all maternal deciduas prior to placing into RPMI media containing streptomycin, nystatin and fetal calf serum.
Amniotic fluid 16-18 weeks
20 ml of amniotic fluid should be taken and divided between two sterile universals. Approximately 10 ml should be referred to a cytogenetics laboratory for karyotyping and culture. The cultured cells serve as a back up in the event that DNA analysis fails to give a clear result. The remaining 10 ml should be sent directly to the PND Unit for DNA analysis.
Fetal blood sampling 18+ weeks
2 ml of blood anticoagulated with EDTA is required.
All fetal samples MUST be accompanied with maternal and paternal blood samples anticoagulated with EDTA (10-15 mls), which will be tested alongside the fetal sample.
Consent forms for DNA testing must be provided with the samples. Testing will not take place without these.
Please contact the PND unit prior to performing fetal sampling.
Blood Sciences Laboratory
Ground Floor Bessemer Wing
King’s College Hospital
London SE5 9RS
Last updated: 08/01/2020