d-dimer Assay

Activation of the coagulation process will lead to the cleavage of fibrinogen to fibrin. The fibrin molecule that results will be cross-linked via D-domain by Factor XIII, producing a stable fibrin clot. Exacerbation of this process can lead to major clot and thrombi formation, which, if not controlled, can be fatal. Activation of the fibrinolytic system results in the conversion of plasminogen into the active protease, plasmin. Plasmin cleaves fibrinogen and fibrin into fragments D and E. Plasmin will release fibrin degradation products with cross-linked D-domains, the smallest unit of which is the D-dimer. Thus, detection of D-dimers is an indication of reactive fibrinolysis. Elevated levels of D-dimer is indicative of the presence of a clot and has been reported in certain pathological conditions such as Pulmonary Embolism (PE), Deep Vein Thrombosis (DVT), and Disseminated Intravascular Coagulation (DIC).
Reference range: 

Normal range: <500ng/ml FEU

Sample type and Volume required: 
One tri-sodium citrate sample.
Turnaround time: 
2 hours maximum
Storage and transport: 
If the assay will not be performed the same day, double spin citrate sample, remove the plasma and store in a capped plastic tube in –40°C freezer. This is stable for 1 month. Thaw for 10 minutes at 37°C, and perform the assay within 2 hours.
Please contact Business Development for pricing enquiries
Time limit for extra tests: 
4 hours
Routine Coagulation - Blood Sciences at King's College Hospital
020 3299 2434
King's College Hospital
Bessemer Wing
Denmark Hill
London SE5 9RS
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 07/08/2015