Red Cell Elution Studies

Elution is the procedure by which we are able to recover any antibody bound to the surface of red blood cells. Eluates prepared from red cells in vivo may be used for the following procedures:
1. To identify antibodies coating the red cells of patients who have a positive Direct Antiglobulin Test (DAT) post transfusion
2. To aid in the investigation of suspected cases of HDN (Haemolytic Disease of the Newborn) either as a result of ABO incompatibility between mother and neonate or as a result of the presence of maternal antibody.
3. To isolate specific antibodies from sera containing multiple specificities by in vitro adsorption to selected red cells.
4. To determine the presence of weakly expressed antigens on red cells after prior incubation with selected anti-sera
5. To prepare specific anti-sera free from unwanted antibodies.
Eluates may also be tested for the presence of specific proteins by a variety of immunochemical or biochemical techniques
Reference range: 


Sample type and Volume required: 
6ml K2EDTA
Call in advance: 
Turnaround time: 
6 hours
Time limit for extra tests: 
1 day
Blood Transfusion Laboratory at King's
Bessemer Wing
King's College Hospital
Bessemer Wing
Denmark Hill
London SE5 9RS
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 07/08/2015