Red Cell Elution Studies
1. To identify antibodies coating the red cells of patients who have a positive Direct Antiglobulin Test (DAT) post transfusion
2. To aid in the investigation of suspected cases of HDN (Haemolytic Disease of the Newborn) either as a result of ABO incompatibility between mother and neonate or as a result of the presence of maternal antibody.
3. To isolate specific antibodies from sera containing multiple specificities by in vitro adsorption to selected red cells.
4. To determine the presence of weakly expressed antigens on red cells after prior incubation with selected anti-sera
5. To prepare specific anti-sera free from unwanted antibodies.
Eluates may also be tested for the presence of specific proteins by a variety of immunochemical or biochemical techniques
King's College Hospital
London SE5 9RS
Last updated: 07/08/2015