Prothrombin fragment 1+2

Concentration of prothrombin fragment 1+2 (PF1+2) is ascertained with a sandwich ELISA. PF1+2 in test plasma is captured by antibodies bound to microtitre plate wells, incubated, and unbound material then washed off. A solution of antibody to PF1+2 that is conjugated to an enzyme is added to ‘tag’ onto any captured PF1+2. Unbound conjugate is washed off and a substrate for the enzyme is added, the product of the enzyme-substrate reaction being coloured. Colour intensity is in direct proportion to the degree of conjugate-binding, itself proportional to the amount of PF1+2 capture and thus, concentration.
Clinical details: 
Assembly of the prothrombinase complex on the activated platelet surface occurs when FXa binds to surface phospholipid via Gla domain-calcium interactions, in association with its co-factor FVa. The substrate, prothrombin, is converted to the serine protease thrombin via cleavage in two locations, Arg271 and Arg320. The first cleavage at Arg271 produces fragment 1+2 and the intermediate prethrombin 2, the fragment 1+2 being released as an activation peptide. Prethrombin 2 is cleaved at Arg320 to yield active thrombin.

Detection of elevated levels of prothrombin fragment 1+2 is indicative of increased thrombin generation and a hypercoagulable state."
Reference range: 

69 - 229

Sample type and Volume required: 
External requests: Citrated platelet poor plasma
375µL x 1 aliquot
Internal requests: please refer to EPR label
Turnaround time: 
14 - 21 days
Special sample instructions: 

The sample should be analysed or manipulated & stored in the laboratory within 4 hours of venepuncture. Please ensure sample tubes are filled exactly to the fill-line as underfilling creates a dilution error and leads to inaccurate results.

Diagnostic Haemostasis and Thrombosis Department
020 7188 2797
St Thomas' Hospital
North Wing - 4th and 5th Floors
Westminster Bridge Road
London SE1 7EH

Laboratory opening times
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 09/03/2017