Plasma Cell Dyscrasias(PCD)

TESTS AVAILABLE: FISH on separated Plasma Cells (CD138+ve)
Diagnostic and relapsed Multiple Myeloma: TP53 (17p13.1); 1q21/1p32.3; IGH-FGFR3 t(4;14); IGH-MAF t(14;16);
Minimum Myeloma panel : If insufficient cells available for a full panel , a minimum of two probes will be applied in the order stated previously.
Clinical details: 
At diagnosis aim is to distinguish between monoclonal gammopathy of undetermined significance, asymptomatic (smoldering) MM, symptomatic MM, solitary plasmacytoma, and other plasma cell diseases. Symptomatic MM is defined as the presence of 10% clonal bone marrow plasma cells and organ damage (hypercalcemia, renal failure, anaemia, or bone lesions [CRAB]). In addition, the presences of 60% bone marrow involvement or rapidly climbing paraprotein, regardless of CRAB, are considered as MM-related symptoms. The diagnostic work-up should include three subsequent levels of investigation to confirm the diagnosis, assess the prognosis, and establish the appropriate treatment. The International Staging System (ISS) is used to assess the prognosis of patients with symptomatic MM (CRAB). Chromosomal abnormalities IGH/FGFR3 t(4;14), IGH/MAF t(14;16), and IGH/MAFB t(14;20); chromosome1 abnormalities (del1p or gain of 1q); and del17p (TP53) detected by fluorescent in situ hybridization (FISH) are associated with poor prognosis. The isolated 13q deletion is not considered a high-risk feature. Hyperdiploidy, t(11;14), and t(6;14) are considered standard-risk features. The combination of FISH data with ISS stage improves risk assessment.
Sample type and Volume required: 
Bone Marrow Aspirate- first draw or/and Blood in case of circulating PC present.
Turnaround time: 
Please refer to Cytogenetics User Guide
Special sample instructions: 

All samples must be delivered to the laboratory within 24 hours of collection.

HMDC Department at King's College Hospital
020 3299 9000 ext 32414
c/o Central Specimen Reception
Blood Sciences Laboratory
Ground Floor Bessemer Wing
King’s College Hospital
Denmark Hill
London SE5 9RS
Mon-Fri, 9.00am-5.30pm
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 03/07/2017