Paul Bunnell Test

The Paul Bunnell test is used to screen for infectious mononucleosis (IM), which is seen most commonly in adolescents and young adults. IM is caused by the Epstein-Barr virus. This infects B-lymphocytes which subsequently produce a heterophile antibody which is identified through its reaction with the Paul Bunnell antigen and is detected by a latex agglutination method.
Clinical details: 
The Paul Bunnell test is not always positive in IM. For higher sensitivity please request an EBV IgM assay.
Reference range: 


Synonyms or keywords: 
IM, Monospot, Infectious Mononucleosis, Glandular Fever, Mono
Sample type and Volume required: 
Whole blood / Serum
Turnaround time: 
1 day
Storage and transport: 
Blood Sciences Department-Guy's And St Thomas' Hospital
Result Query: 020 7188 8008
St Thomas' Hospital
North Wing - 5th Floor
Westminster Bridge Road
London SE1 7EH
Contact: 020 7188 9247

Guy's Hospital
Southwark Wing - 4th Floor
Great Maze Pond
London SE1 9RT
Contact: 020 7188 4781
Haematology Laboratory at Guy's and St Thomas' Clinical Advisory Service
Routine hours: bleep 1358
Paediatrics Routine hours: bleep 1621

Out of hours, weekend and bank holidays: 17:00-9:00 bleep 0294
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 07/08/2015