Parvovirus DNA

Qualitative real-time Polymerase Chain reaction (PCR) assay for detection of Parvovirus DNA. Used for the diagnosis of Parvovirus infection. Test can be performed by Virology on basis of clinical details provided or by contacting Virology StR and test requested on EPR as "sample for molecular investigations" and sending EDTA blood.

Clinical details: 
Qualitative PCR and/or Viral load testing may be appropriate in some situations. Eg
- Sickle cell/haemoglobinopathy patient presenting with aplastic crisis during prodrome, before rash/arthropathy and serological response develop.
- Immunocompromised patients present with anaemia (or pancytopenia), without rash/arthropathy/detectable parvovirus specific antibodies.
- Pregnant woman with red rash exposure or fetal abnormalities on ultrasound. Parvo IgM may be short lived, with only parvo IgG detected despite primary infection in pregnancy.
Reference range: 

Qualitative - Detected/ Not detected

Synonyms or keywords: 
Reticulocyte count Pregnancy Hydrops fetalis intrauterine transfusion Haemoglobinopathy Sickle cell disease Immunocompromised host FSGS
Not applicable
Sample type and Volume required: 
4-9ml Purple Top EDTA blood (for whole blood or plasma), 2.5 – 5ml Yellow Top Serum Separation Tube (SST) Clotted Blood (for serum), amniotic fluid, post mortem heart swabs in universal transport media (UTM) or cerebrospinal fluid (CSF) in a sterile universal container with no additives.
Turnaround time: 
7 days
Storage and transport: 
Place labelled sample in a sealed sample bag and send to Central Specimen Reception (CSR). Samples should be sent to CSR promptly as the sensitivity of assays that detect RNA/DNA depend on sample quality/integrity. Please store samples in a refrigerator if there is a delay in sending samples to CSR.
Infection Sciences Department at St Thomas' Hospital
020 7188 8008
St Thomas' Hospital
North Wing - 5th Floor
Westminster Bridge Road
London SE1 7EH
Core opening hours: Monday-Friday 0900-1730

For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 25/11/2017