The Kleihauer test is performed in the following situations: Rh D negative women delivered of Rh D positive, 'weak D’, Du, D variant, D mosaic or D category infants or other known Rh D epitope variants. Rh D negative women delivered of infants of unknown Rh D type Rh D variant, mosaic or category women who deliver Rh D positive infants. Rh D negative women more than 20 weeks pregnant, having a sensitising event. Delivery of an Rh D Positive or D variant infant. Abortion after 20 weeks gestation. Invasive prenatal diagnosis- Amniocentesis, Chronic villus sampling, Fetal blood sampling, other intrauterine procedures. Insertion of shunts. Embryo reduction- Significant ante partum haemorrhage (APH), not normal 'spotting'. External version of Foetus (ECV). Closed abdominal injury. Ectopic pregnancy. Intrauterine death (IUD). Stillbirth. Miscarriage at > 20 weeks. Occasionally in cases of Intra Uterine Death of unknown cause where mother is Rh D positive. If the patient has recurrent bleeding a Kleihauer is performed at 2 weekly intervals, with anti-D being issued at 6 weekly intervals.
Reference range: 


Sample type and Volume required: 
6ml K2EDTA
Call in advance: 
Turnaround time: 
1 day
Time limit for extra tests: 
1 day
Blood Transfusion Laboratory at King's
Bessemer Wing
King's College Hospital
Bessemer Wing
Denmark Hill
London SE5 9RS
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 07/08/2015