HIV-1 CCR5 tropism prediction

Description: 
In house assay that genotypes the envelope V3 loop of HIV-1 gp120.
Clinical details: 
HIV infects cells expressing the primary receptor CD4 and one of the chemokine receptors CCR5 or CXCR4. CCR5 antagonists, such as the anti-retroviral drug Maraviroc, inhibit replication of R5 virus. Tropism testing is essential prior to use of this drug in order to indentify individuals harbouring X4 or R5/X4 variants of HIV in whom no significant virological response to Maraviroc treatment would be anticipated.
Reference range: 

Not applicable. Result given as predicted tropism

Synonyms or keywords: 
HIV, Tropism, genotyping, Maraviroc, sequencing
Units: 
Predicted co-receptor use categorised as; CCR5 (R5), CXCR4 (X4), dual tropic (R5/X4) or mixed tropic (R5,X4,R5/X4).
Sample type and Volume required: 
10 ml EDTA plasma, CSF
Call in advance: 
For samples with a viral viral load <1,000 copies/ml
Turnaround time: 
2 weeks
Storage and transport: 
Room temperature or refrigerated if there is a delay in transport
Contacts:
South London Specialist Virology Centre
Results: 02032996155 Medical Advice: 02032996298
King's College Hospital
Cheyne Wing, 2nd Floor (opposite Liver ITU)
Denmark Hill
London SE5 9RS

DX address: South London (PHL) Kings DX 6570200 Peckham 90SE
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 07/08/2015