Fibrinogen antigen is measured in a sandwich ELISA. Fibrinogen in test plasma captured by antibodies bound to microtitre plate wells, incubated, and unbound material then washed off. A solution of antibody to fibrinogen that is conjugated to an enzyme is added to ‘tag’ onto any captured fibrinogen. Unbound conjugate is washed off and a substrate for the enzyme is added, the product of the enzyme-substrate reaction being coloured. Colour intensity is in direct proportion to the degree of conjugate-binding, itself proportional to the amount of fibrinogen capture and thus, concentration.
Quantitative and qualitative deficiencies of fibrinogen can be congenital or acquired and give rise to bleeding. More rarely, some dysfibrinogenemias can predispose to thrombosis.
1.97 - 4.76
400µL x 1 aliquot
Internal requests: please refer to EPR label
The sample should be analysed or manipulated & stored in the laboratory within 4 hours of venepuncture. Please ensure sample tubes are filled exactly to the fill-line as underfilling creates a dilution error and leads to inaccurate results.
North Wing - 4th and 5th Floors
Westminster Bridge Road
London SE1 7EH
Laboratory opening times
Last updated: 09/03/2017