Deceased donor crossmatch (on-call)

Flow cytometry analysing IgG binding to donor T and B lymphocytes from peripheral blood, lymph node or spleen.
Clinical details: 
In solid organ transplantation the presence of HLA donor specific antibodies is associated with an increased risk of graft loss and graft dysfunction. Flow cytometric (FCXM) crossmatch tests are used to identify HLA donor specific antibodies. A crossmatch test is performed in deceased kidney, kidney pancreas transplantation and pancreatic islet transplantation.
Reference range: 

sensitised: >2.3 - positive unsensitised: >4.0 - positive

Synonyms or keywords: 
DCD DBD crossmatch urgent donor spleen lymph node PBL
Sample type and Volume required: 
2cm piece of spleen, Lymph nodes or 30ml EDTA blood
Call in advance: 
On call staff must be contacted by the designated person responsible for transplantation to request this test prior to sending samples. On call staff must be contacted by a person from Richard Bright Ward as soon as the samples arrive
Turnaround time: 
5 hours
Special sample instructions: 

Sample must be sent to Richard Bright Ward at Guy's Hospital

Storage and transport: 
Temperature controlled
Time limit for extra tests: 
Clinical Transplantation Department at Guy's Hospital
Laboratory Enquiry: 020 7188 1540
Clinical Transplantation Department
Guy's Hospital
Borough Wing - 3rd Floor
Great Maze Pond
London SE1 9RT
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 07/08/2015