TB investigation respiratory samples
Description:
Bronchial lavage (BAL), induced sputum, non-directed bronchial lavage, sputum for mycobacteria investigation.
Microscopy (auramine staining for AFB ) and culture (BD MGIT and LJ slopes) is performed in Viapath Analytics St Thomas's Microbiology. Positive cultures are referred to the National Mycobacterium Reference Laboratory for identification and sensitivity testing.
Turn around time microscopy 1-2 days, culture 8-10weeks (8 weeks for a negative culture).
Significant auramine and culture results are telephoned to the ward or clinical team by Microbiology Clinical staff.Microscopy, isolation of Mycobacteria.
Microscopy (auramine staining for AFB ) and culture (BD MGIT and LJ slopes) is performed in Viapath Analytics St Thomas's Microbiology. Positive cultures are referred to the National Mycobacterium Reference Laboratory for identification and sensitivity testing.
Turn around time microscopy 1-2 days, culture 8-10weeks (8 weeks for a negative culture).
Significant auramine and culture results are telephoned to the ward or clinical team by Microbiology Clinical staff.Microscopy, isolation of Mycobacteria.
Clinical details:
Mycobacterial infection, TB, AFB
Synonyms or keywords:
AFB, auramine stain, CSF, ascitic fluid, pleural fluid.
Department:
Location:
Sample type and Volume required:
Bronchial lavage for AFB, induced sputum for AFB, ND-bronchial lav for AFB, sputum for AFB.
Please send 5 ml in a plain sterile universal container.
Please send 5 ml in a plain sterile universal container.
Call in advance:
For samples that requires an urgent auramine, please ring the laboratory on 83111 during core hours and bleep out of hours (17.00-9.00) on bleep number 1802.
Turnaround time:
Turn around time microscopy (auramine) 1-2 days, culture 6-10 weeks (6 weeks for a negative culture). Significant auramine and culture results are telephoned to the ward or clinical team by Microbiology Clinical staff.
Storage and transport:
Place labelled sample in a sealed sample bag and send to Central Specimen Reception (CSR). Samples should be refrigerated if transport to the laboratory is delayed.
Contacts:
Infection Sciences Department at St Thomas' Hospital
020 7188 8008
St Thomas' Hospital
North Wing - 5th Floor
Westminster Bridge Road
London SE1 7EH
Core opening hours: Monday-Friday 0900-1730
North Wing - 5th Floor
Westminster Bridge Road
London SE1 7EH
Core opening hours: Monday-Friday 0900-1730
Laboratory:
Last updated: 01/10/2020
