LKM1 blot (Part of liver immunoblot panel)
Description:
Part of Liver immunoblot panel, includes M2, LKM, LC-1 and SLA antibodies.
Antibodies to liver and kidney microsomes are characteristically associated with Type 2 autoimmune hepatitis. They may develop transiently after viral hepatitis. The antibody is tested for by IIF using a rodent composite block of liver, kidney and stomach. Sub-types of anti-LKM have been described, anti-LKM 1, 2 and 3, based on the IIF staining pattern. It can be difficult to detect in the presence of anti-gastric parietal cell antibody and requires experience to be able distinguish these patterns. The laboratory routinely reports anti-LKM 1 antibodies and this done after confirmation by autoimmune liver blot and a titre result issued.
Antibodies to liver and kidney microsomes are characteristically associated with Type 2 autoimmune hepatitis. They may develop transiently after viral hepatitis. The antibody is tested for by IIF using a rodent composite block of liver, kidney and stomach. Sub-types of anti-LKM have been described, anti-LKM 1, 2 and 3, based on the IIF staining pattern. It can be difficult to detect in the presence of anti-gastric parietal cell antibody and requires experience to be able distinguish these patterns. The laboratory routinely reports anti-LKM 1 antibodies and this done after confirmation by autoimmune liver blot and a titre result issued.
Reference range:
Negative / positive.
Units:
Negative - equivocal - positive
Department:
Location:
Sample type and Volume required:
Clotted (Gold top vacutainer)
Turnaround time:
6 days
Special sample instructions:
All tests are compromised by prolonged transit times. For this reason, date of bleed and date of postage must be provided with each request.
Storage and transport:
Samples may be stored at 4°C prior to despatch. The use of first class letter post without the need for coolant is sufficient.
Time limit for extra tests:
10 days
Contacts:
Diagnostic Immunology and Allergy Department at King's College Hospital
General Enquiry : 020 3299 8752 / 020 3299 1171
King's College Hospital
Bessemer Wing - 1st Floor
Denmark Hill
London SE5 9RS
Bessemer Wing - 1st Floor
Denmark Hill
London SE5 9RS
Laboratory:
Last updated: 01/03/2019
