Anti-Adalimumab Antibodies (Free)

Description: 
The measurement of anti-Adalimumab antibodies enables a greater understanding as to the underlying aetiology of treatment failure. Patients with detectable anti-Adalimumab antibodies may be at risk of developing hypersensitivity reactions, particularly those being re-introduced after drug interruption. The detection of anti-Adalimumab antibodies also guides management decisions on concomitant immunosupression , dose escalation, drug switching and withdrawal.

Measured as part of a profile including Adalimumab levels.
Clinical details: 
Due to the structure of the anti-TNFα drugs, the immune system may stimulate formation of antibodies against the drug (ADA, anti-drug antibodies). Antibody formation is associated with allergic reactions and loss of response due to neutralisation of biological effect and/or increased clearance of drug. Adalimumab, being a fully humanised monoclonal antibody, is thought to be less immunogenic than Infliximab.

Reference range: 

<10 ng/mL Please note that in the presence of drug, anti-drug antibody may not be detected. Test interpretation requires full clinical information: drug dosage/frequency, previous exposure to other biologics, time of sampling, duration of therapy and clinical assessment.

Units: 
ng/mL
Sample type and Volume required: 
Serum or plasma. Collect blood into a serum separation (SST™), plain or lithium heparin tube. Minimum 300μL serum or plasma required for both drug and anti-drug antibody analysis.
Turnaround time: 
2 weeks
Special sample instructions: 

Collect blood into a serum separation (SST™) or plain tube, preferably shortly before drug administration (trough levels). Centrifuge sample at 3000 rpm for 10 minutes, aliquot serum or plasma and keep in fridge until transport. If transport is going to be delayed by > 5 days, freeze at -20°C and send by first class post at a later date.

Storage and transport: 
Post the sample to GSTS Pathology by first class post.
Contacts:
Reference Chemistry Laboratory at St Thomas'
0207 188 1264
4th floor, North Wing
St Thomas' Hospital
Westminster Bridge Road
London SE1 7EH
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 19/02/2020