Colorimetric dye-binding assay for the quantitative determination of albumin in human serum.
Albumin is a small globular protein with a molecular mass of 66.3 kDa, which constitutes 55-65 % of total plasma protein. It maintains plasma oncotic pressure, and is also involved in the transport and storage of a wide variety of ligands and is a source of endogenous amino acids. Albumin binds and solubilises various compounds, e.g. bilirubin, calcium and long-chain fatty acids. It is capable of binding toxic heavy metal ions as well as numerous pharmaceuticals, which is the reason why lower albumin concentrations in blood have a significant effect on pharmacokinetics.